OPERATION IN EXPERT MODE
LSM 510
DuoScan
Carl Zeiss
Acquire Menu
LSM 510 META
DuoScan
4-68
B 45-0021 e
03/06
(4)
Beam path - META detector settings
On the Beam Path and Channel Assignment panel use the two sliders to define the spectral detection
range of the META module. The current position of the slider is displayed in a white box when keeping
the left mouse button pressed.
Both slider values are updated in the
Start
and
End
Input box.
The wavelength values can also be typed directly into the Start and End input boxes. The position of the
sliders are updated after the next mouse click. The
Number of Passes
displays the number of successive
scans to be performed in order to cover the required spectral range.
Step
check box:
Step size can be increased to 21.4 nm, 32.10 nm or 42.80 nm by binning of 2, 4
or 8 adjacent channels, respectively.
4.5.3.9
Settings in the Online Finger-
printing Mode
The use of this function permits the selection of
reference spectra together with the excitation
settings, to allow an immediate display of the
unmixed images while scanning.
•
Click on the
Config
button in the
Acquire
subordinate toolbar of the main menu.
−
The
Configuration Control
window opens.
•
Click on the
Online Fingerprinting
button.
−
The
Beam Path and Channel Assignment
panel for the
Online Fingerprinting
mode
is opened.
a
Beam Path configuration
Activation / deactivation of the excitation wavelengths (check box) and setting of
excitation intensities (slider). Open the
Laser Control
window via the
Laser
button. The active laser lines are automatically displayed in the wavelength color
bar.
Selection of the main dichroic beam splitter (HFT) through selection from
Fig. 4-48
Configuration Control window;
Online Fingerprinting Mode activated
