LSM 510
OPERATION IN EXPERT MODE
LSM 510 META
Acquire Menu
Carl Zeiss
03/06
B 45-0021 e
4-71
4.5.4 Scan
Control
The scan parameters for image acquisition are set
in the
Scan Control
window.
The microscope must be in the LSM mode, i.e. the
LSM
button on the tube of the microscope stand
must be pushed or the
LSM
button in the
Acquire
subordinate toolbar must be activated to set the
LSM mode.
The scanning actions are started via the buttons on
the right-hand side of the
Scan Control
window,
and the scan parameters are set in the main part of
the window.
An acquired image is displayed in a separate
Image Display
window. If an
Image Display
window is not yet available, a new
Image Display
window is automatically opened during the
acquisition.
The following scanning modes can be performed:
Spot
−
scanning of a spot (Spot + Time Series)
Line
−
scanning of a line in the XY-plane (Line, Line + Time Series)
−
scanning of a line with different Z-values (Line + Z Stack, Line + Z Stack + Time Series)
Frame
−
scanning of an XY frame (Frame, Frame + Time Series)
−
scanning of XY frames with different Z-values (Frame + Z Stack, Frame + Z Stack + Time Series)
−
scanning of XY frames in defined ROIs (Frame + Use ROI + Time Series)
−
scanning of XY frames with different Z-values in defined ROIs (Frame + Z Stack + Use ROI + Time
Series)
Fig. 4-50
Scan Control window
