LSM 510
OPERATION IN EXPERT MODE
LSM 510 META
Acquire Menu
Carl Zeiss
03/06
B 45-0021 e
4-83
The parameters of a Z Stack can be defined using the
Z Sectioning
tab, the
Mark First/Last
tab or - if
the optional Piezo focus for objectives is connected - the
Hyperfine Z Sectioning
tab.
Z Sectioning
tab
Num Slices
:
Entry of the number of sections (single XY-images) to be recorded with the stack via
the slider / arrow buttons. The entry does not influence the interval.
Interval
:
Entry of the step width (Z-distance between the single XY-images) via slider / arrow
buttons. The entry has no influence on
Num Slices
.
Current Slice
:
Display of the current position of the slice within the stack. Change of position via
slider / arrow keys. Reset of the current slice position in the center of the stack by
clicking on the
C
button. Of course, the borders of the stack are also changed if the
current slice position is changed.
Keep Interval
:
The interval remains constant when the stack limits or number of slices are changed.
Keep Slices
:
The number of slices remains constant when the stack limits or interval are changed.
The Stack Z Size is slightly adjusted to hold the number of slices with no
interval changes or the interval size if the number of slices is varied.
Fast Z Line
:
Not available for frame mode. Fast Z scan for overviews (only for
Line
scan mode).
The stack size is retained; the interval is adapted depending on the scan speed.
Determining the optimum stack size with the help of the Line Sel and Range function
•
Click on the
Line Sel
button.
−
An XY-scan of the current slice is performed. The cutline is displayed in the image center. The
Line
toolbar is displayed on the right-hand side of the
Image Display
window.
Fig. 4-64
Scan Control window - Z Sectioning tab activated
