LSM 510
OPERATION IN EXPERT MODE
LSM 510 META
Acquire Menu
Carl Zeiss
03/06
B 45-0021 e
4-119
4.5.7.2 Settings
Panel
The
Settings
panel allows you to determine when,
where and how the bleaching process shall be
done (only works in connection with time series).
Furthermore, all the settings of the
Bleach
Control
window can be stored, reactivated or
deleted in this panel.
a
Bleach after
number scans
:
If this check box is ticked , the bleaching procedure is automatically performed
in combination with a time series. Under
Scan Number,
you must enter after
how many scanning procedures bleaching is to be performed.
Scan Number
:
Number of Scans in a time series, after performance of which the bleaching
procedure shall be started.
Bleach repeat after
number scans
:
If this check box is ticked , the bleaching procedure is automatically performed
in combination with a time series. Under
Scan Number,
you must enter after
how many scanning procedures bleaching is to be repeated.
Scan Number
:
Number of Scans in a time series, after performance of which the bleaching
procedure shall be repeated.
Different Z
Position
:
If this check box is ticked , you can set the current stage position as the one in
which the bleaching will be done by clicking the
Mark Position Z
button. This
function is only available using the
Line
or
Frame
scanning mode.
Simultaneous Grab
and Bleach
(Only available at
systems equipped
with LSM
DuoScan
.)
If this check box is ticked , only the laser lines form the LSM
DuoScan
are
available and the bleach procedure will be done simultaneously to the imaging
procedure that is set up in the scan control window. For the imaging the
configuration set up in the configuration control menu is used. The bleach
procedure is performed according to the set up in Bleach Control window. It is
not possible to chose a different Z-Position for bleaching when this function is
activated.
Different Scan
Speed
If this check box is ticked , the speed for scanning during the bleach process
can be defined independently of the scan speed during image acquisition. A
lower speed results in a longer pixel dwell time which increases the efficiency of
bleaching.
Fig. 4-98
Settings panel
