LSM 510
OPERATION IN EXPERT MODE
LSM 510 META
Macro Menu
Carl Zeiss
03/06
B 45-0021 e
4-187
•
Adjust the AOM Transmission to keep the power indicated at the power meter constant, then press
Add and + 10 steps
again.
•
Repeat this procedure until you have acquired the AOM settings for all wavelengths; 10 nm steps are
sufficient.
The step size for tuning can be edited. Choosing
Manual
(checkbox) requires to perform the
tuning using the slider or typing in a value. Single values or the whole list can be deleted.
Normalize
list
will set the highest value to 100 and adjust the others accordingly.
Recommended for all data. Mean intensity (%) refers to the overall image intensity value; The
value is displayed as % of the maximum intensity value at chosen data depth (8 or 12 bit).
Calibration files are stored as *.aom files in a folder of choice. Scanning can be started and
stopped using the buttons
Scan Cont
and
Stop Scan
on the right side of the Macro Window.
It is recommended to use a power meter with sub-mW resolution to obtain best results.
(2) Using a Mai Tai Laser:
In the lower part of the window the calibration table is shown together with the file currently used, the
laser status, objective and main dichroic; The latter will influence the laser power in the sample -> use
calibration curves acquired with the same settings as image acquisition is performed.
The store wavelength button will update the laser wavelength in the main SW.
(3) Using a Chameleon Laser:
In the lower part of the window the calibration table is shown together with the file currently used, the
laser status, objective and main dichroic; The latter will influence the laser power at the sample -> use
calibration curves acquired with the same settings as image acquisition is performed. When tuning at
whatever process you are performing the option Wait for optimization can be set as desired.
Fig. 4-181
Example using a Mai Tai Laser
