LSM 510
OPERATION IN EXPERT MODE
LSM 510 META
Macro Menu
Carl Zeiss
03/06
B 45-0021 e
4-189
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Advanced Linear Unmixing:
The algorithm used for Linear Unmixing calculates the minimal
difference between the unknown spectra and the reference spectra for all possible mixtures of the
reference spectra (lowest square fit of acquired spectrum). This can produce negative values for the
contribution of individual spectra. Advanced Linear Unmixing ignores the negative values and starts
a new calculation based on the remaining spectra. (Without performing Advanced Linear Unmixing,
these values will be set to zero).
−
Ask for Background ROI:
Subtracts a spectrum defined by a ROI in the acquired Lambda Stack
before performing the Linear Unmixing. This parameter is suitable to remove homogenous
background signals.
−
Ignore overexposed and underexposed pixels:
Pixels displaying above the highest or lowest
intensity level will not be included in calculations.
−
Generate Channel with Residuals:
Generates an additional „Channel with
Residuals“ which is a pixel-by-pixel display of
the difference between fit and original data
(for the channel of the Lambda Stack that
shows the greatest deviation) Perfect fit for a
given pixel is indicated by a pixel with
intensity 0 in the corresponding channel
displaying the residuals.
Fig. 4-183
Channel with Residuals
