OPERATION IN EXPERT MODE
LSM 510
DuoScan
Carl Zeiss
Macro Menu
LSM 510 META
DuoScan
4-190
B 45-0021 e
03/06
4.8.4.6 Concatenate
Images
System requirements:
−
LSM 5 system, software release 3.5 or higher
This program combines open image files (single images, z-stacks, time series) to a single file series.
Macro Panel:
Add (Click into image window):
Click
Add
button followed by a click in the open image window. An
image link will appear in the row of icons below (time line). The palette and the channel settings will be
transferred. Overlays will only appear in concatenated images in RGB- mode.
Remove:
Removes the active image link out of the concatenate image row.
Remove All:
Clears the concatenate image row.
Repeat:
Adjusts the number of image copies to be used in the concatenated time series. Repeated image
copies can e.g. be used for adjustment of playback frame rate when creating a movie. (If a movie plays a
frame rate of 15 images/sec and if an image is supposed to be seen for 2 seconds the repeat slider needs
to be set at 30 image copies.)
Mode:
Selects the options of data storage. Two options are possible: RGB e.g. can be used for exporting
data and making movies. Raw image data e.g. can be used for further image processing and
concatenating time data in image series.
−
Concatenating images in RGB mode:
All Images from time series, stacks and single images are
concatenated in one series regardless of data depth (Channels, Z-depth, slice number).
−
Concatenating images in Raw image data mode:
Intersection mode: The number of channels in the
concatenated image series depends on the image with the fewest channels. The color of the
channel is adjusted depending on data of the first concatenated image in the row. XYZC data is
treated similar.
Size
selection: Selects the image size adjustment by concatenation of image data.
−
Intersection:
Adjusts the image size to a common form (In RGB mode e.g. concatenate frame size
256x 256 with 512x 512, frame size 256x 256 will be adopted to 512x 512 pixel by stretching)
−
Union:
Adopts the established image sizes (in this case 256x 256 frame size being concatenated
with a frame size of 512x 512, both image sizes will be shown in the series).
−
Fixed size:
Width and height of the frame size can be set. Square images will be modified according
to the smallest side of the frame (e.g. width is set to 300pixel and height is set to 250pixel, a
square image will be shown in a frame size of 250x 250pixel).
Apply button:
Linked images in the icon row will be concatenated to a time series image stack.
Close button:
Closes the Concatenate Images macro window.