LSM 510
OPERATION IN EXPERT MODE
LSM 510 META
Display and Analysis of Images
Carl Zeiss
03/06
B 45-0021 e
4-273
(2)
PSF tab (optional with 3D DCV)
The objective data are displayed in the
PSF
tab. In
the case of wavelengths above 700 nm, the
NLO
button is automatically enabled.
The displayed values are always taken over by the
system data, but can be edited subsequently for
simulation purposes.
•
Select the required method and determine the
relevant parameters.
The deconvolution calculation is performed
immediately after the
2D Deconvolution
window
has been closed, and the image display is updated
(on-line).
4.13.18
Display - Cut
This function allows to
−
display a Z Stack of images at a user defined
section plane (= cut plane)
−
improve the image of the section plane by
trilinear interpolation
The settings of
Chan
,
Zoom
,
Slice
,
Contr
and
Palette
are applied.
•
Clicking on the
Cut
button in the
Display
toolbar opens the
Cut
to the right of the
Image
Display
window.
Any changes done with this toolbar are
effective immediately. The content of the
overlay plane is temporarily deleted while the
toolbar is displayed.
•
By varying the parameters
X
,
Y
,
Z
,
Pitch
and
Yaw
, you can position a section plane of any
orientation within the stack volume.
•
The resulting position of the section plane is
shown as a red area below the
Trilinear
Interpolation
button. At the same time, the
result is shown in the
Image Display
window.
•
A click on the
Reset All
button restores the
original position.
•
A click on the
Trilinear Interpolation
button
will improve the quality of the image by
performing a 3D interpolation of the image.
Fig. 4-268
PSF tab
Fig. 4-269
Image Display window, Cut display
