OPERATION IN EXPERT MODE
LSM 510
DuoScan
Carl Zeiss
Image Optimization
LSM 510 META
DuoScan
4-354
B 45-0021 e
03/06
As a rule, the first scanned image (Pre-Scan) is not ideal, since the photomultiplier is not matched to the
light output. More often than not, the screen image is dull and needs subsequent optimization.
•
Click on the
Channels
button in the
Scan Control
window.
−
This opens the Channel Settings and Excitation of Track panels.
−
The channels used are color-highlighted.
4.14.2.2
Image Optimization
The image optimization processes
−
setting of pinhole diameter
−
Detector Gain / Ampl. Offset / Ampl. Gain
−
Scanning speed and Average
must be carried out separately for each channel used (see
Single Channel
, page 4-345).
For the optimum setting of the single channels,
Split xy
-display must be selected in the
Image Display
window to enable the direct viewing of the separate images of the relevant channels.
•
Click on the
Cont.
button in the
Scan Control
window.
−
This starts a continuous scan.
•
Click on the
Split xy
button in the
Image Display
window toolbar.
−
This displays the separate images scanned in the channels and the composite (overlay) image.
Fig. 4-335
Scan control and Image Display windows
