Routine Mode
LSM 510
DuoScan
Carl Zeiss
Application of Examination Methods in …
LSM 510 META
DuoScan
6-10
B 45-0021 e
03/06
(1)
Routine Mode - Image Stack Setup
window
The buttons arranged on the right in the
Image Stack Setup
window have the following functions:
New Image
button
Opens a new image window.
Start
button
Triggers scanning of a stack.
Stop Scan
button
Stops the current scan procedure.
XYscan
button
Triggers single XY-scan.
XYcont
button
Triggers continuous XY-scan.
Stage
button
Opens the Stage and Focus Control window.
The definition of the stack parameters in the
Image Stack Setup
window is made in accordance with
the exported Examination Method. If the exported method was created using the PIEZO 200 fine
focusing stage, the Z-parameters are defined via the
Line Sel
/
Range
function of the
Hyperfine Z
Sectioning
panel.
Otherwise, use the
Mark First/Last
function for the definition of the Z-parameters.
Mark First/Last function
The parameters of the Z Stack to be created are
also displayed in the window and can be changed
online.
Stack Z Size
:
Optical indication, i.e. the optical
beam moves over this (displayed)
Z-thickness (online). The stage
(nosepiece) is moved in such a
way that the stack size,
dependent on the refractive
index, is achieved optically.
Focus Position
: Mechanical indication, i.e. if the
refractive index changes, the
value of the focus position also
changes in relation to the "0"
value (online).
Z Sectioning
: Setting
of
Number of Slices
,
Interval
and
Current Slice
via
sliders / arrow buttons.
Mark First/Last
: Determination of the Z-value for
the first and last XY-image of
the stack, combined with
manual focusing or
Stage
control.
Fig. 6-13
Image Stack Setup window
(Mark First/Last)
