3D FOR LSM
LSM 510
DuoScan
Carl Zeiss
Overview and Explanations
LSM 510 META
DuoScan
7-2
B 45-0021 e
03/06
7
3D FOR LSM
7.1
Overview and Explanations
7.1.1
The Image Sequence
The "3D for LSM" handles image sequences
generated by the Zeiss LSM software. This can be
three-dimensional image data or a time sequence
of two-dimensional images (slices). Each slice (as
well as the sequence) can consist of up to eight
channels. An image sequence consists of a series
of individual (2D) images and has a name that
designates the entire sequence. In general an
image sequence is handled as a single object in the
system. Individual channels or slices can be
addressed.
The following terms and definitions apply for the
"3D for LSM" software.
−
An image sequence is a number of individual
sequential images (usually called slices in the
dialog boxes), the spacing between which is
equal.
−
Image sequences can contain up to 12 bit of
image data (per channel).
−
A sequence (slice) can consist of up to eight
channels.
−
The maximum size of an image sequence is
limited by the provided memory of the
operating system.
−
A voxel is the smallest element of an image sequence (the equivalent of a pixel in a 2D image). All
voxels in a given image sequence are the same size.
−
The coordinate system originates in the left upper front corner of the image sequence. This point has
the coordinates 0, 0, 0.
−
All angles are positive for rotations to the right in the direction of the positive coordinate axis (right-
handed coordinate system).
−
A slice is an individual image in a sequence of images. The numbering of the slices starts with "1".
Image sequences can consist of several channels. Most functions and the
Display window
are providing
buttons to select all or a subset of channels stored in the selected image sequence. The
Output
image
sequence will only get those channels which are selected on the input side. The button selects all
channels in the image sequence to be used clicking with the left mouse button on it.
Clicking with the left mouse button on any of the number buttons toggles the state of this single
channel.
Clicking with the right mouse button on any of the number buttons selects this single channel exclusively.
All other channels are deselected.
Single slice with
single channel
Image sequence
Multichannel
(0, 0, 0)
Single slice with
multiple channels
Voxel
Y
X
Z
Positive rotation directions
of the axes
Fig. 7-1
