LSM 510
ANNEX
LSM 510 META
Non Descanned Detection
Carl Zeiss
03/06
B 45-0021 e
8-29
•
Click on the
Non-Descanned Detection
button to change to Non-Descanned Detection.
•
Configure the NDD channels analog to the
Descanned Detection mode.
The configuration of multitracks is also
possible for NDD applications, though not
in combination with the standard
channels.
•
Before activating image acquisition close the
shutter in front of the HBO or HAL illumination.
Push in the pushrod (8-15/
1
) for closing the
shutter. This enhances image quality. If the
hardware is still 3.0 or lower make sure that the
detector gain for any used NDD is set to the
minimal value before opening the manual
shutter in front of the HAL / HBO illumination.
•
Switching from LSM to VIS mode automatically
stops scanning. Open the shutter in front of the
HBO or HAL illumination for conventional
florescence or transmitted light microscopy.
Be aware that the NDD reflector cube in the reflector turret leads to a strong back reflection of
HBO light into the specimen plane and the eyepiece. When observing the specimen through the
ocular lens the use of the NDD reflector cube should be avoided. The light flash is not harmful
but unpleasant. To avoid this an additional filter (#1261-345) can be mounted into the NDD
reflector cube which greatly reduces the intensity of the back reflected light in the eyepiece. This
filter reduces the intensity of the excitation light by 7 %.
Fig. 8-15
Non descanned detection module
with HBO 100 lamp
