INTRODUCTION TO LASER SCANNING MICROSCOPY
LSM 510
DuoScan
Carl Zeiss
Optical Diagram of the LSM 510
LSM 510 META
DuoScan
3-4
B 45-0021 e
03/06
3.3
Optical Diagram of the LSM 510 and LSM 510 META (Schematic)
Fig. 3-2
Optical path, schematic (4-channel configuration)
AOTF Acousto Optical Tunable Filter
DBC
Dichroic Beam Combiner
DBS
Dichroic Beam Splitter
EF Emission Filter
HAL Halogen
Lamp
HBO Mercury Vapor Short-Arc Lamp
LSF
Line Selection Filter
MDBS Main Dichroic Beam Splitter
NDF
Neutral Density Filter
VP
Variable Pinhole
PMT Photomultiplier
T-PMT Transmission-Photomultiplier
The diagram above is a schematic representation of the LSM system.
Laser light is focused onto the specimen through an objective in a diffraction-limited mode. Light emitted
at the focal plane and at planes below and above it is directed via an XY scanner onto a main dichroic
beam splitter (MDBS), which separates the emissions from the excitation light. The fluorescences are
separated from each other by a series of dichroic beam splitters (DBS1 ... maximally DBS4) and directed to
individual photomultipliers (PMT1 ... maximally PMT4).
