03/06
13
Image optimization
Choosing a lookup table
•
Select
Palette
in the
Image
window of the
scanned image (Fig. 19).
The
Color Palette
window appears.
•
In the
Color Palette List
panel, click on the
Range Indicator
item (Fig. 20).
The scanned image appears in a false-color
presentation (Fig. 19).
If the image is too bright, it appears red on the
screen. Red = saturation (maximum).
If the image is not bright enough, it appears blue
on the screen. Blue = zero (minimum).
Adjusting the laser intensity
•
Set the
Pinhole
to
1
Airy Unit (Fig. 21).
•
Set the
Detector Gain
high.
•
When the image is saturated, reduce AOTF
transmission in the
Excitation
using the slider to reduce the intensity of the
laser light at the specimen.
Adjusting gain and offset
•
Increase the
Amplifier Offset
until all blue
pixels disappear, and then make it slightly
positive (Fig. 21).
•
Reduce the
Detector Gain
until the red pixels
only just disappear.
Fig. 19
Image window
Fig. 20
Color Palette window
Fig. 21
Scan Control window, Channel settings
