14
03/06
Scanning a Z stack
•
Select
Z Stack
in the
Scan Control
window.
•
Select
Frame
if necessary.
The
Z Settings
panel appears.
•
Select
Mark First/Last
on the
Z Settings
panel.
•
Click on the
XY cont
button.
A continuous XY-scan of the set focus position will
be performed.
•
Use the focusing drive of the microscope to
focus on the upper position of the specimen
area where the Z Stack is to start.
•
Click on the
Mark First
button to set the upper
position of the Z Stack.
•
Then focus on the lower specimen area where
the recording of the Z Stack is to end.
•
Click on the
Mark Last
button to set this lower
position.
•
Click on
X:Y:Z=1:1:1
button to set the Z-
interval in such a way that the voxel has
identical dimensions in the X-, Y- and Z-
directions (cube).
•
Click on the
Start
button to start the recording
of the Z Stack.
Fig. 22
Scan Control window, Z Stack settings
