LSM 510
OPERATION IN EXPERT MODE
LSM 510 META
Acquire Menu
Carl Zeiss
03/06
B 45-0021 e
4-51
Microscope settings on Axiovert for reflected-light observation (Epi-fluorescence)
•
Turn on the HBO 100 power supply switch (4-32/
1
).
•
Click on the
Reflected Light
button and set the shutter in the
Open
position.
•
Click on the
Reflector
button and select the desired filter set by clicking on it.
•
The filter is automatically moved into the beam path to enable observation in epi-fluorescence.
•
Click on the
Tube Lens
button and select the tube lens.
•
Click on the
Objective
button and select the objective.
If the X-Cite 120 fiber coupled lamp for
reflected-light illumination is used a
further dialog is opened when clicking the
Reflected light button. The lamp internal
shutter and the attenuation function can
be controlled via the LSM Software. The
lamp has to be switched on first using the
main switch on the lamp housing.
Remote
Enables the attenuation control via LSM Software. This is also enabled
when clicking the
ON
button.
ON
Opens the shutter in the reflected light path and the internal shutter of
the lamp. Both shutters are closed automatically when switching to LSM
mode.
Shutter
Opens and closes the
shutter in the reflected light path independent of
the status of the internal lamp shutter and the attenuation.
Intensity
Sets the light via input box or slider.
Lamp Hours
Indicates the total time the lamp has been in use.
4.5.2.4
Working in LSM Mode
Switchover to the scanning mode is then required.
•
Click on the
LSM
button in the
Acquire
subordinate toolbar.
4.5.2.5
Microscope Control for Axioskop 2 FS
MOT
For setting the Axioskop 2 FS
MOT
, proceed in the same way as with Axio Imager.Z1 and Axiovert 200 M.
Since the Axioskop 2 FS
MOT
is not motorized (except the external Z drive and the tube), all microscope
settings have to be made manually.
Especially, the change of objectives is made manually. The used objective must be set in the
Scan
Control
window.
Fig. 4-33
X-Cite 120 control panel
