OPERATION IN EXPERT MODE
LSM 510
DuoScan
Carl Zeiss
Acquire Menu
LSM 510 META
DuoScan
4-122
B 45-0021 e
03/06
4.5.7.5
Start / End a Bleaching Procedure
•
The bleaching process will be started via the
Bleach
button. Only the bleach protocol set up will be
executed but no image is displayed. It is also possible to start the bleaching process via the
Bleach
button in the
Time Series Control
window or to combine it with a time series and hence with
imaging. To perform the latter it is necessary to define the number of images to be acquired before
bleaching and to define the region for bleaching.
When a trigger key is activated to start the bleaching procedure, the
Waiting for Trigger
message first
appears in the status line of the
Bleach Control
window. In that case, the bleaching procedure is started
after activation of the relevant trigger key.
•
The bleaching process can be finished via
Stop
in the
Bleach Control
window.
Stop
does not only stop the bleaching process, but the entire scanning process.
4.5.8 Stage
The following software description applies to
systems which are equipped with a motorized
stage.
This window enables you to activate both the
motor focus and the scanning stage.
The
Focus Position
and
Stage Position
panels
include the function keys for the performance of
defined moves and the display of the current Z and
X, Y positions.
By use of an LSM 510 META scanhead
on an Axiovert 200 M sideport system
care should be taken when moving the
motorized XY scanning stage to the
maximum positions, so that fingers are
not bruised between scan head and
stage.
Fig. 4-102
Stage and Focus Control window
