Zeiss LSM 700, Specifications

Microscopes

Stands

Upright: Axio Imager.Z1m, M1m and Axio Scope mot for LSM

Inverted: Axio Observer.Z1m SP (side port) 

Z drive

Axio Imager: Step motor, smallest increment 10 or 25 nm
Axio Observer: DC motor with opto-electronic coding, smallest increment 10 nm

XY stage (option)

Motorized XY scanning stage with Mark & Find (XYZ) and Tile Scan (Mosaic Scan) functions

Objectives

More than 40 reflected-light objectives: EC Epiplans, EC Epiplan-Neofluars, EC Epiplan-Neofluars, 
EC Epiplan-Apochromats, LD Epiplans, LD EC Epiplan-Neofluars

Accessories

High-resolution AxioCam microscope camera

Scanning module

Scanner

Two independent galvanometric scanning mirrors with ultra-short line and frame flyback

Scanning resolution

4 x 1 to 2048 x 2048 pixels, continuously adjustable

Scanning speed

13 x 2 speed stages, Up to 5 frames/s with 512 x 512 pixels (max. 154 frames/s with 512 x 16 pixels)

Scanning zoom

0.5 x to 40 x, variable in increments of 0.1

Scanning rotation

Freely rotatable around 360°, variable in increments of 0.1°

Scanning field

Field diagonal of 18 mm (max.) in the intermediate image plane, homogeneous illumination

of image field

Pinhole

Motorized master pinhole, diameter continuously adjustable

Detection

One or two confocal channels (reflection/fluorescence), one optional external transmitted-light

channel with DIC capability, each with high-sensitivity PMT detector, spectral increment 1 nm

Data depth

Selectable between 8 bit, 12 bit or 16 bit

Laser inserts 

Laser inserts (VIS, V)

Pigtail-coupled solid-state laser with polarization-preserving single-mode fiber; up to 4 V/VIS

laser directly connectable to the scanning module; laser lines 405 nm 5 mW or 445 nm 5 mW;

488 nm 10 mW; 555 nm 10 mW; 639 nm 5 mW (at fiber end)

Fast (pixel-exact) customized and variable intensity adjustment of all laser lines (direct modulation)
Automatic shutdown of laser when not in use

Electronics module

Control computer

Real-time electronics integrated in PC; communication with user PC via PCI express; Control of 
microscope, lasers, scanning module and additional accessories, data acquisition and synchronization;
Over sampling acquisition for best sensitivity and doubled SNR; possibility of online data-analysis 

during acquisition

User PC

High-end PC with ample RAM and hard disk storage capacity; ergonomic high-resolution 
16:10 LCD-TFT flat-panel display, Windows VISTA operating system with multi-user capability 
Ethernet connection to local network 

Standard software

System configuration 

Convenient control of all motorized microscope functions, laser modules, and scanning module, 

saving and restoring of application configurations 

ReUse function 

Restoration of acquisition parameters per mouse click

Capture modes 

Spot, Line/Spline, Frame, Z Stack, Time-Lapse Series and combinations:

XY, XYZ, XYT, XYZT, XZ, XT, XZT, Spot-T,

Averaging and summation (line-wise or frame-wise, configurable)

Step Scan (for higher frame rates, configurable) 

Crop function 

Convenient selection of scanning ranges (simultaneous zoom, offset and rotation)

Spline scan 

Scanning along a freehand defined line

Image processing

Image processing options for any kind of computations, addition, subtraction, multiplication, 

division, ratio, shift, filters (low-pass, median, high-pass, etc., also user-definable) 

Presentation

Orthogonal view (XY, XZ, YZ in a single presentation) Cut view (3D section made under a freely

definable spatial angle) 2.5D view for time-lapse series of line scans Projections (stereo, maximum, 
transparency) for single frames and series (animations) Depth coding (pseudo-color presentation of 
height information) Brightness and contrast adjustments; off-line interpolation for Z stacks Selection

and modification of color lookup tables (LUTs), drawing functions for documentation

Analysis

Intensity profile measurement of straight lines and curves of any shape Measurement of lengths,

angles, areas, intensities, etc. 

Data archiving

ZEN file browser with convenient functions for managing experiments together with their

acquisition parameters Multiprint function for creating assembled image and data views. 

More than 20 file formats (TIF, BMP, JPG, PSD, PCX, GIF, AVI, Quicktime, etc.) for compatibility

with all common image processing programs 

Optional software

Topography package

Computation and visualization of surfaces (fast rendering modes) and height profiles,

plus many measurement functions (roughness, surface area, volume)

StitchArt plus package

Capture of multiple XZ profiles and multiple XYZ stacks with reflected light

LSM Image VisArt plus

Fast 3D and 4D reconstruction and animation (shadow and transparency projection, surface

and mixed rendering modes, cutting planes, fly-through mode, distance measurement in 3D)

3D for LSM

3D presentation and measurement of volume data records, on request

3D Deconvolution

Image restoration based on computed point spread functions with fluorescence

(Modes: nearest neighbor, maximum likelihood, constrained iterative) 

Freeware 

Image Browser 
ZEN LE

Free software packages for display, processing, sorting, printing

and 

Export/import of LSM 5/7 images

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