Zeiss LSM 700, Instructions Manual

p.

16



Save

the

image



The

intensity

data

from

the

ROI

can

be

viewed

in

the

ROI

mean

tab

in

the

image

window

Note:

The

region

can

be

defined

in

other

types

of

experiments

with

or

without

time

lapse

and

can

also

be

used

as

a

tool

of

zooming

in

by

checking

Fit

Frame

size

to

bounding

rectangle

of

regions.

Positions



A

positions

list

can

be

added

to

a

multidimensional

experiment

in

which

all

of

the

settings

will

be

acquired

(channel,

acquisition

mode,

Z

stack,

tile,

time

series).



The

Positions

tab

can

be

a

useful

tool

in

managing

a

large

tile

and

for

quick

slide

scanning

(via

the

ocular)

with

low

magnification

before

moving

to

high

magnification

with

small

field

of

view.



Add

a

position

by

pressing

Positions

below

the

image

container

and

clicking

on

the

image

container

that

was

just

acquired

at

the

current

stage

position



Check

Positions

at

the

Multidimensional

Acquisition

options.



At

the

Positions

tab

move

to

a

position

in

the

list

by

selecting

and

pressing

Move

to



A

position

can

also

be

added

by

pressing

Add

in

the

Positions

tab

Note

:

If

you

use

the

positions

list

only

to

locate

saved

positions

uncheck

Positions

from

the

Multidimensional

Acquisition

options

before

pressing

Start

Experiment,

otherwise

your

settings

will

be

acquired

at

every

position

in

the

list.

Note

you

can

use

multiple

options

in

the

multidimensional

scanning

for

example

Z

stack

&

tile

Time

series,

bleaching,

ROI

&

Z

stack

and

positions.