p.
6
Sample
viewing
In
the
Locate
tab
choose
the
preferred
objective
filter
set
and
open
the
illumination
shutter
RL\TL
Set
the
Transmitted
light
(In
case
the
TL
is
needed)
Click
the
transmitted
light
lamp
icon,
click
ON
and
set
desired
brightness
(or
via
the
microscope).
Set
the
filter
turret
position
to
DIC
and
the
condenser
below
the
stage
to
I,
II
or
III
according
to
the
objective
lens
(see
objective
lens
info
on
touchpad).
If
you
are
acquiring
transmitted
channel
set
up
Kohler
illumination:
1.
Bring
the
sample
into
focus.
2.
Close
the
field
stop
using
the
motorized
aperture
button
(F)
located
at
the
right
of
the
microscope
until
you
can
see
at
least
one
edge
3.
Adjust
the
condenser
height
until
the
edges
of
the
diaphragm
image
are
crisp.
4.
Center
the
diaphragm
image
using
the
two
centering
screws.
5.
Open
the
field
diaphragm,
just
until
the
image
fills
the
field
of
view.
Summary of Contents for LSM 700
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