p.
9
Select
show
all
tool
and
open
the
Imaging
set
up
tab
Note:
make
sure
that
the
chosen
configuration
is
correct
and
suits
the
fluorophores;
make
changes
if
needed
it
is
highly
recommended
to
check
all
dyes
spectrums
in
a
spectra
viewer
ThermoFisher
Chroma
1.
Laser
lines
2.
Main
dichroic
mirror
3.
PMTs:
PMT1
and
PMT2
4.
Variable
secondary
dichroic
beamsplitter
(VSD).
5.
Emission
Filter
selection
6.
Emission
spectrum
with
Graphic
representation
of
the
laser
line,
the
position
of
the
VSD
and
emission
filter.
7.
Transmitted
light
PMT
‐
a
transmitted
light
image
(non
confocal)
can
be
added
to
the
confocal
image
(it
recommended
to
add
to
the
longest
wavelength
track).
1
2
3.2
4
5
6
7
3.1
Summary of Contents for LSM 700
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