8
6.
Several methods are available :
-FASTEST : allows a simultaneous acquisition ; it is the fastest method but the cross talk from one
color to the next channel could be important.
-BEST SIGNAL : each channel is acquired separately. It is the slowest method but it efficiently avoids
the Cross talk phenomenon.
-BEST COMPROMISE : compromise between speed and reduction of Cross talk
Example : blue and red channels are acquired simultaneously, then the green one is acquired alone.
-LINEAR UNMIXING : use spectral capacity of the system to separate simultaneously the different
dyes. First, you need to acquire sample stained with only one color.
Tricks :
These modes are a help to start your configuration. The software does not always make the
smartest choice . You have to check by yourself and then make some modifications.
