25
This monograph comprehensively deals with the
quality parameters of resolution, depth discrimina-
tion, noise and digitization, as well as their mutual
interaction. The set of equations presented allows
in-depth theoretical investigations into the feasibili-
ty of carrying out intended experiments with a
confocal LSM.
The difficult problem of quantifying the interaction
between resolution and noise in a confocal LSM is
solved by way of the concept of resolution proba-
bility; i.e. the unrestricted validity of the findings
described in Part 1 is always dependent on a suffi-
cient number of photons reaching the detector.
Therefore, most applications of confocal fluores-
cence microscopy tend to demand pinhole diameters
greater than 0.25 AU ; a diameter of 1 AU is a
typical setting.
Summary
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