LEFT TOOL AREA AND HARDWARE CONTROL TOOLS
LSM 710 and LSM 780
Carl Zeiss
Acquisition Tab
Systems
58 M60-1-0025
e
02/2010
Within the
Imaging Setup
tool (Fig. 47)
information on the laser line and detection band is
displayed if the arrow left of the
activation/deactivation tick box of each track is
clicked. The white line represents the laser line and
the color bar(s) represents the detection band for
the channel(s) in this track. If a track is not
activated it will be ignored for image acquisition.
Tracks can be added and subtracted by clicking
plus or minus. The added
Track
has the same filter
settings as the first track and one active channel.
With the
Show all
mode active, it is possible to
store a track under a name. A stored track can be
chosen from the drop down menu and can be
loaded into the highlighted profile of a track. Thus
stored tracks can easily be integrated into other
imaging configurations.
Lambda Mode:
The
Lambda Mode
(Fig. 48) is chosen for imaging
heavily overlapping emission signals. When
choosing this mode in the drop down menu the
display changes to show the currently active laser
line(s) and the detection range of the QUASAR
detector.
With this imaging mode, it is possible to acquire an
intensity image displaying the intensity of the
fluorochrome(s) within a spectral bandwidth of
10 nm. According to the detection range that is set
for the QUASAR detector, a specific number of
such images is acquired which is called a Lambda
stack.
These image data provide the intensity information within the selected detection range for each pixel.
Therefore, the data allow deducing an emission spectrum for each pixel corresponding to the emission
spectrum of a specific dye.
This calculation is done by linear unmixing (see section
Unmixing View
) using the acquired Lambda
stack and allow to clearly separate even heavily overlapping emission signals.
Fig. 47
Imaging Setup tool: Channel Mode
Fig. 48
Imaging Setup tool: Lambda Mode
