LEFT TOOL AREA AND HARDWARE CONTROL TOOLS
LSM 710 and LSM 780
Carl Zeiss
Acquisition Tab
Systems
60 M60-1-0025
e
02/2010
5.2.5
Tool Group Setup Manager: Light Path Tool
This tool displays the hardware settings of the scan head (
LSM
tab), the beam path to image with the
camera (
Camera
tab) or the Non descanned Detectors (
Non Descanned
tab). The settings can be
changed in the corresponding tab and used for a new imaging configuration. The configuration can be
stored in the
Imaging Setup
tool.
The
Channel Mode
tab
(Fig. 50) displays the
current hardware settings within the LSM 710 and
LSM 780 system's scan head for an imaging track.
The display changes in accordance with the
selection of a track in the
Imaging Setup
tool.
It also allows accessing the focus control, the stage
position (the latter is only available if a motorized
XY stage is attached to the microscope) and the
laser lines. Fig. 50 shows the
LSM
tab when
channel mode is selected in the
Imaging Setup
tool including a description of the various icons.
(1)
Main dichroic beam splitters (MBS)
•
MBS stands for main dichroic beam splitter. This
beam splitter is chosen according to the
selected laser line(s). It separates the excitation
light from the emission signal. HT filters reflect
the wavelength indicated and transmit all other
wavelengths.
•
To select a beam splitter, click on the icon and
chose the desired one from the drop down
menu.
(2) Detection
sliders
To select an emission filter, click on the sliders and set the detection bands according to the dry emission
spectra on the top.
For the configuration of the beam path, please refer to the application-specific configurations
depending on the used dyes and markers and the existing instrument configuration listed in the
annex.
Fig. 50
Light Path tool
