LEFT TOOL AREA AND HARDWARE CONTROL TOOLS
LSM 710 and LSM 780
Carl Zeiss
Acquisition Tab
Systems
64 M60-1-0025
e
02/2010
5.2.5.7
Imaging in Lambda Mode
(to be selected in the
Imaging Setup
tool or in the
Light Path
tool).
The
Lambda Mode
is recording the overall
emission from the sample onto a wavelength-
dispersive element and is imaged on a 34-channel
detector. All 34 photomultipliers of the detector
cover a spectral width of approximately 360 nm, a
single PMT covers a spectral range of 9.7 nm. In
the
Lambda Mode
, images, image stacks, or time
series are recorded in a wavelength selective way.
This new image format is called Lambda Stack. For
the acquisition of a Lambda Stack, the complete
fluorescence signal can be read out at once. The
settings of the beam path for the
Lambda Mode
scanning procedure with regard to the main
dichroic beam splitter and the QUASAR detector
settings are performed in the
Light Path
tool
(Fig. 54).
The QUASAR detector is displayed for spectral
imaging if lambda mode has been chosen as
imaging configuration in the
Imaging Setup
tool.
The
Light Path
tool displays the laser lines, the
main (dichroic) beam splitter, and the spectral
range of the QUASAR detector.
You can change the settings using the following function elements:
Activation / deactivation of the excitation wavelengths (check box) and setting of
excitation intensities (slider).
Selection of the main dichroic beam splitter (MBS) through selection from the
relevant list box.
Definition of number of PMTs out of the 34 PMT Detectors to fit the required
spectral range.
Access to the focus control (see section
Tool Group Online Acquisition: Focus
Tool
)
Step width in spectral range (9.7 recommended).
Activation / deactivation of reflection.
Fig. 54
Light Path tool window;
Lambda Mode activated
