LEFT TOOL AREA AND HARDWARE CONTROL TOOLS
LSM 710 and LSM 780
Carl Zeiss
Acquisition Tab
Systems
68 M60-1-0025
e
02/2010
5.2.6.1
Frame Mode for LSM
When the scan mode
Frame
is active, a frame of
variable size is scanned pixel by pixel and line by
line. The laser beam is moved over the specimen
line by line.
The following additional parameters and be set:
Objective, Frame Size, Line Step Factor, Speed,
Pixel Depth, Scan Direction, Averaging and
Zoom including rotation and offset
A list of available objectives is displayed in the
scroll down menu next to
Objective
. Choose one
from the list which is appropriate for imaging a
specific specimen. Pay attention to match the
refractive index of the objective with the refractive
index of the embedding medium of the specimen
if possible.
•
Select the
Frame Size
from the default sizes via
the drop down menu clicking the button
or type in the required values next to X and Y.
Recommended setting to start with: 512 x 512
pixels.
−
It is also possible to enter different values for
X and Y. The value for
Y
is freely selectable
between 1 and 6144 pixels (integers). The
value for
X
must always be an integral
multiple of 4. The maximum value for
X
is
also 6144 pixels.
−
The
Optimal
button sets the image
resolution to an optimal value corresponding to the optical magnification (objective) the zoom and
the emission range detected. This provides an image where no information is lost as optimal
sampling is achieved.
•
Select the
Line Step
size between 1 and 10. Only every n-th line is scanned. The lines in between are
interpolated. This fast scan mode is called Step Scan.
•
Select the
Scan Speed
from the 15 preset steps via slider or input box. Recommended: 8 for the first
scan. A click on the
Max
button sets the maximum speed for the current zoom.
−
The
Scan Speed
determines the
Pixel Dwell
time. In the case of different image formats, the
Pixel Dwell
time is constant for the same Scan Speed, but the
Scan Time
is different.
−
Pixel Dwell
time of the laser beam on the pixel
−
The
Scan Time
duration of the acquisition for the entire frame
Speed:
1
...
9
10 11 12 13 14 15
Zoom:
0.6-0.7 0.8-1.2 1.3-1.9 2.0-3.0 3.1-4.9 5.0-8.3 >
8.4
Mode*:
lbda./ch.
lbda./ch.
24-ch
16-ch 8-ch 4-ch 2-ch
* recommendation; depends on system configuration, pixel resolution and bit depth.
Fig. 57
Acquisition Mode - Frame with LSM tab
selected in the Light Path tool
