LSM 710 and LSM 780
LEFT TOOL AREA AND HARDWARE CONTROL TOOLS
Systems
Acquisition Tab
Carl Zeiss
02/2010 M60-1-0025
e
81
5.2.8
Ratio Channels
The parameters for ratio metric imaging can
be set when a ratio channel is selected.
•
Click on the button of a ratio channel
(e.g.
R1
).
•
Clicking on the required tabs enables you
to choose from four formulas (
Type 1
to
4
) for ratio calculation. The relevant
decimal values can be entered in the
input boxes. The entered values remain
unchanged even after switchover to
another formula and can be reactivated
any time.
The formula type activated last is always
used for ratio formation during the scan
procedure. If the input box does not contain
any value at all or no suitable value, the
useful value last used will be activated.
The ratio channels are displayed as additional images.
Select the required formula and enter the relevant values.
Letters can be entered into the formula fields which will be valued as 1; it is also possible to make no
entry, which will also be valued as 1, but will not be displayed.
Set by min/max allows the definition of the display scaling according to the expected minimal and
maximal values.
•
Laser lines and laser power are displayed according to each track. It is possible to vary laser intensities
(in the same way as in the
Laser
tool or
Light Path
tool) and to program the AOTF for different laser
lines using the Switch button.
All parameters can be varied while imaging.
Fig. 72
Channel Settings panel of a Ratio Channel
