LEFT TOOL AREA AND HARDWARE CONTROL TOOLS
LSM 710 and LSM 780
Carl Zeiss
Acquisition Tab
Systems
84 M60-1-0025
e
02/2010
5.2.11
Tool Group Online Acquisition:
Regions Tool
The
Regions
tool allows the user to define Regions
of Interest (ROI) which are used for image
acquisition, sample manipulation (bleaching) and
image analysis (Fig. 75).
The control of the laser via AOTF technology
enables the restriction of the illumination to the
defined region. The sample is only illuminated
when data are acquired.
The definition of the regions is done using an
actual image of the sample and drawing the
regions into this image. This makes it extremely
easy to set the regions in the right position and
dimension.
The following tools are available:
Selects an existing region in the image. The outline of the region is marked with
small rectangles to show it is active. The format and the position of the region can be
changed clicking on the rectangles or the region outline and dragging it with the
cursor. In addition the numerical values describing the position and size of the region
are updated (see below).
Rectangle
: Drawing of a rectangle in the
Image
window; click and keep mouse
button pressed, drag the rectangle in any direction, let go off the mouse button to
end the procedure.
Ellipse
: Drawing of an ellipse in the
Image Display
window; first click sets the
center point, displayed line permits determination of the extension, second click sets
the first dimension, then the second dimension and the rotation direction can be
determined, third click sets the second dimension and direction and ends the
procedure.
Circle
: Drawing of a circle in the
Image
window; click and keep the mouse button
pressed to set the center point, drag the diameter, let go off mouse button again to
end the procedure.
Polyline
: Drawing of a polyline figure in the
Image Display
window; first click sets
the starting point, each further click adds a line, double-click on the starting point
closes the figure and ends the procedure.
Closed
Bezier
: Drawing of a bezier figure in the
Image
window; first click sets the
starting point, each additional click adds a line, double-click on the starting point
closes the figure and ends the procedure.
Fig. 75
Regions window
