LEFT TOOL AREA AND HARDWARE CONTROL TOOLS
LSM 710 and LSM 780
Carl Zeiss
Acquisition Tab
Systems
92 M60-1-0025
e
02/2010
Use one out of four triggers with
Trigger In
to trigger the bleaching process. In addition, one out of
four triggers can be used as
Trigger Out
signal to control an external device. (For a detailed description
on the trigger use and interface see printed manual CHAPTER 7 ANNEX).
If a region is used to define a position for bleaching within the sample, the bleach process can be
accelerated by checking the box next to
Zoom Bleach (fast, less accurate)
. In this case the scanner
movement will be restricted to the bleach region zooming in onto this region. This may result in a less
accurate positioning of the region as the definition of the region has been made in a different zoom in
the image. The bleach process will be faster than without this option. The gain in speed is dependent on
the speed that is used for bleaching. If a high speed has already been chosen for bleaching the gain in
speed will be minimal.
Define the region(s) for bleaching in the
Regions
tool (see section
Tool Group Online Acquisition:
Regions Tool
). Only a region activated under
Bleach is considered for bleaching. In the
Excitation of Bleach
area (Fig. 83) chose the laser
line and laser intensity which is then used for
bleaching.
It is possible to choose different laser lines and/or
laser intensities for the bleach process of each
region when activating the option:
Use different
Settings for different ROIs
. A maximum number
of six regions is possible. The numbers of the
regions correspond to the numbers indicated next
to
ROI#
. For each region, a tab is active which
allows setting individual laser parameters for
bleaching.
Fig. 83
Excitation of Bleach area for setting
laser line and intensity for bleaching
