LEFT TOOL AREA AND HARDWARE CONTROL TOOLS
LSM 710 and LSM 780
Carl Zeiss
Acquisition Tab
Systems
94 M60-1-0025
e
02/2010
The markers visible in the image series, have different colors with the following meaning:
•
red
:
manually set marker with time indication and comments
•
blue
:
automatically set marker with change of delay
•
green
: automatically set marker at the beginning and at the end of a bleaching procedure
The acquisition of a time series is started using the
Start
action button.
Pre-Scan
If this box is checked, a continuous scan is performed when clicking
Start
, but no
images are acquired until you hit the button
. This button becomes
visible next to
Pause
in the lower part of the control window.
Alternatively, you can start the acquisition using a
Trigger
In
or using the system
Time
of the computer.
In any case it is possible to send a
Trigger Out
signal with the start of the times series.
When starting a Time Series via Trigger or Time the message
Waiting for Trigger
or
Waiting for Start
Time
will then be displayed in the status line when hitting
Start
.
Pause
This function interrupts the Time Series. The labelling changes to
Resume
. The
current scan finishes with the end of the frame or line. When Resume is clicked,
the Time Series is immediately continued.
The same options as for starting a time series apply for ending a time series.
Focus Stabilizer
This check box activates the Definite Focus unit.
If the time interval between two time points is below 11s – the focus
stabilizer will not be activated between the two time points.
If the entered number of cycles is processed without a set trigger impulse been given to end the
procedure, the Time Series is finished.
If a trigger signal to end the scan arrives before the cycles have been processed, the Time Series
will only be interrupted.
Waiting for Trigger
will be displayed in the status line. The Time Series
can now be continued via a new trigger signal or ended via
Stop
.
A Time Series is finished when the end
Time
is reached.
If the entered number of
Cycles
has been processed, the Time Series is finished before the end
Time. If the number of cycles has not yet been processed until the end Time, the Time Series is
also finished. The end time for the Time Series can be changed online. Bleach times will be
added to the total time for image acquisition.
If a time series is interrupted before its programmed end, the programmed number of images
will be taken over in the database. However, only those images are stored which were created
before interruption of the time series. This is due to the fact that the original image parameters
are to be taken over via the
Reuse
function.
