LSM 710 and LSM 780
LEFT TOOL AREA AND HARDWARE CONTROL TOOLS
Systems
Acquisition Tab
Carl Zeiss
02/2010 M60-1-0025
e
101
•
Activate channel 1 via the check box. You may
want to assign a different color to the channel
which will be taken over for the initial display of
the image. Click the channels icon to open the
color table and select the desired color (Fig. 97).
•
Move on the slider to set a detection window
above.
•
If required, deactivate all the other channels
(ChS, Ch2, 4, monitor diode, transmission,
R1-2) via check box.
•
Click on the icon of the main dichroic beam
splitter and select
MBS 488/561
or any other
MBS indicating the 561 line.
The beam path in the
Light Path
tool now displays
the way the potential emission signal takes.
Channel 3 receives the red part of the spectrum.
The settings are suitable for imaging dyes like
Rhodamin, TRITC or Cy3.
For overlaying fluorescence and transmitted-light
images, click on the
T-PMT
(Transmission) button
in the
Beam Path and Channel Assignment
panel.
All transmitted light applications like
−
phase contrast
−
differential interference contrast (DIC)
−
polarization contrast (Pol)
−
darkfield
can be performed if the hardware of the
microscope is equipped with the necessary optics.
We recommend DIC as standard contrast because
the suitable objectives have a higher transmission
than the ones for phase contrast. This enhances
detection sensitivity.
In case intensity fluctuations occur e.g. with
405 nm lasers, a different attenuation level should
be chosen. In case this is not possible, a different
laser line needs to be selected for the T-PMT
acquisition or track.
Fig. 97
Channel color selection
Fig. 98
Light Path tool window
