LEFT TOOL AREA AND HARDWARE CONTROL TOOLS
LSM 710 and LSM 780
Carl Zeiss
Processing Tab
Systems
124 M60-1-0025
e
02/2010
5.3.10
Ion Concentration
This Processing function permits the calibration of
ion concentrations in physiological experiments.
To open the
Ion Concentration
tools click
Ion
concentration
(Fig. 131).
The two
buttons select the active image
in the
Image Display
as Input Image and Input
Image 2 (for background subtraction). The name of
the automatically generated Output Image is
shown under the name of the Input Images.
At the bottom of the
Ion concentration
tool
window thumbnails of the input images as well as
a preview of the output image are displayed.
After opening the full window by clicking the little
arrow under the Output Image description
(Fig. 132), the full tool window opens and the
following functions are available (Fig. 133).
Opens the display for the resulting calibration curve.
buttons
To load & save calibration curves from / to files.
tick box
To select Input Image 2 as background image.
Image scaling
Spin boxes to set the Min and Max in the Output Image
Pull down menus for
To set three different calibration options, according to the dyes (single
wavelength, vs ratiometric), the calibration method and if the calibration is
carried out in vitro/situ. Ch 1 and 2 select the channels from Input Image 1.
Fig. 131
Ion Concentration in the Processing tab
Fig. 132
Ion Concentration tool window
