LSM 710 and LSM 780
LEFT TOOL AREA AND HARDWARE CONTROL TOOLS
Systems
Processing Tab
Carl Zeiss
02/2010 M60-1-0025
e
139
5.3.14.3
Channel Shift
Channel Shift
is used to produce a congruent
image with relation to the pixels of the various
channels (Fig. 151).
This pixel correction function is particularly
important in UV applications.
This is a manipulation of your data that
has to be considered very carefully when
co-localization analysis is part of your
experiment.
The
button selects the active image in the
Image Display
as Input Image.
The image has to be a multi-channel image. In the
•
Select the channels you want shift by ticking the
Ch1
or
Ch2
check box:
•
And use the sliders
or the spin-boxes
to select the pixel
shift in the horizontal and vertical direction.
Clicking the Zero next to the spin-boxes resets
the shift to the original position.
−
A preview is automatically generated at the
bottom of the
Channel Shift
tool in the
Processing
tab of the Left Tool Area and at
the same time. A large preview is shown in
the
Preview
view tab in the Image display in
the Center Screen Area.
•
Click on the
Apply
button to generate a new
image with the set pixel shift.
Fig. 151
Channel Shift window
