LEFT TOOL AREA AND HARDWARE CONTROL TOOLS
LSM 710 and LSM 780
Carl Zeiss
Maintain Tab
Systems
152 M60-1-0025
e
02/2010
(3) Hardware
Press the
Load configuration
register to open the
panel.
The
Hardware
tab (see Fig. 171) allows you to set
several hardware defaults.
By activation of the
Lasers off on Exit
check box
the lasers are automatically switched off when the
ZEN software is closed. The lasers are allowed to
cool for five minutes before they are switched off.
The Z-Stack
Waitstate
function is recommended for acquisition with scanning stages, high power
objectives and short acquisition times on Axio Imager stands.
Waitstate
is on as default setting. This
function delays the image acquisition during time series or Z-Stacks in case vibrations of the stage occur
and are registered at the beginning of individual images. The delay is set to 350 ms. This can occur for
specific image acquisition settings and is dependent on the speed, the stage and the objective in use.
Online scanner calibration
(OSC) is on as default setting. This function assures the calibration of the
scanners for bidirectional scanning. If that function is not checked it might be required to calibrate the
scanners in x and y manually when bidirectional scanning is used for image acquisition. Nonlinear
bidirectional distortions especially at high acquisition speeds (10-15) can only be removed by OSC.
(4) Image
display
Any changes in the
Image Display
will per default
not affect the original image data. When the check
box
Apply brightness and contrast to diagrams
and tables
(see Fig. 172) is marked, any changes
in brightness and contrast of the image will also
affect the image data shown in diagrams or tables.
The
Temporary Files Directory
can be set
manually. For large datasets it is advisable to use a
drive
other
than the C-Drive of your computer.
Interpolate
: Interpolates the signal between pixels
and thus smoothens the image. This interpolation
is not saved with the original image data and can
be active also during acquisition.
For the options
Invert x
,
Invert y
,
Invert z
,
Invert time
and
Exchange x and y
the original
data are also not changed when those options are
applied.
Fig.
171
Options tool with hardware panel
opened
Fig. 172
Options tool with image display panel
opened
