CENTER SCREEN AREA / IMAGE CONTAINERS - …
LSM 710 and LSM 780
Carl Zeiss
Colocalization View
Systems
202 M60-1-0025
e
02/2010
6.10.2
Quantitative Colocalization Parameters Shown in the Data Table
−
No. of pixels
in image ROI or scatter region
−
Area / relative area
of image ROI or scatter region
−
Mean intensities / SD
within image ROI or scatter region
−
Colocalization coefficients
−
Weighted colocalization coefficients
−
Overlap coefficient
after Manders
−
Correlation coefficients
(R and R
2
)
6.10.3
Colocalization Coefficients
total
Ch
coloc
Ch
pixels
pixels
c
,
1
,
1
1
=
total
Ch
coloc
Ch
pixels
pixels
c
,
2
,
2
2
=
−
Relative number of colocalizing pixels in channel 1 or 2, respectively, as compared to the total
number of pixels above threshold.
−
Value range 0 – 1 (0: no colocalization, 1: all pixels colocalize)
−
All pixels above background count
irrespective of their intensity.
Weighted colocalization coefficients
∑
∑
=
i
i,total
i
i,coloc
Ch
Ch
M
1
1
1
∑
∑
=
i
i,total
i
i,coloc
Ch
Ch
M
2
2
2
−
Sum of intensities of colocalizing pixels in channel 1 or 2, respectively, as compared to the overall
sum of pixel intensities above threshold and in this channel.
−
Value range 0 – 1 (0: no colocalization, 1: all pixels colocalize)
−
Bright pixels contribute more than faint pixels
Correlation coefficient, Pearson´s correlation coefficient
∑
∑
∑
−
−
−
−
=
i
i
aver
i
aver
i
i
aver
i
aver
i
p
Ch
Ch
Ch
Ch
Ch
Ch
Ch
Ch
R
2
2
)
2
2
(
*
)
1
1
(
)
2
2
(
*
)
1
1
(
−
Provides information on the intensity distribution within the colocalizing region
−
Value range -1 to +1
-1,+1: all pixels are found on straight line in the scatter diagram
0:
pixels in scattergram distribute in a cloud with no preferential direction
