CENTER SCREEN AREA / IMAGE CONTAINERS - …
LSM 710 and LSM 780
Carl Zeiss
FRET View
Systems
236 M60-1-0025
e
02/2010
Settings tab (Fig. 264):
This tab provides access to the definition of the
parameters used for the image analysis.
The
General
tab applies to both methods,
Acceptor Photobleaching and Sensitized Emission.
If checked the listed options will be taken into
account for the analysis of FRET.
The
Acceptor Photobleach
tab (Fig. 265) sets
additional parameters to analyze the Acceptor
Photobleaching experiment such as the choice for
Truncation
(as Remove Max or No Truncation)
and the option not to display negative values in the
image (
Don’t show negative values in the FRET
image
).
6.16.2
Tools in the FRET View Options
Control Block for Sensitized
Emission
FRET tab (Fig. 266):
Within this tab overlay regions can be defined.
(The drawing tools correspond to the drawing
tools described in section
Overlay
).
The check boxes
Numbers
and
Measure
refer to
the overlay regions and annotate the number of
the region and its area to the overlay in the image.
Export
allows the resulting FRET image to be saved as a separate image file including the table with the
results.
The regions defined can be set as
Object
(Region where FRET should be calculated) or
Background
(from which the threshold for the image analysis can be deduced from).
Regions and the assigned status as Object or Background can be selected or deselected for the individual
Analysis using the corresponding check box under Enabled.
As Method for FRET Analysis now three different options are available in the drop down list:
−
Fc (Youvan)
,
−
FRETN (Gordon)
and
−
N-FRET (Xi)
.
Fig.
264
FRET View Options Control Block,
Settings tab, General
Fig.
265
FRET View Options Control Block,
Settings tab, Acceptor Photobleach
Fig.
266
FRET View Options Control Block,
FRET tab
