LSM 710 and LSM 780
CENTER SCREEN AREA / IMAGE CONTAINERS - …
Systems
Unmixing View
Carl Zeiss
02/2010 M60-1-0025
e
239
6.17
Unmixing View
In the
Unmixing View
type, Lambda or channel
data sets can be analyzed and spectrally unmixed.
In particular it allows to:
−
display the spectra corresponding to user-
defined ROIs (mean ROI intensity over
Lambda),
−
use Lambda Stacks and all types of multi-
channel images as input (only if the PMTs of
the LSM system are calibrated),
−
show the intensity values in table form and
copy table to clipboard or save as text file,
−
generate unmixed multi channel images.
The available tools and the functionality of the four
general View Option control blocks
Dimensions
,
Display
,
and
Overlay
is the same as in the
Lambda Coded
View (see the previous section on
this view type). The two additional view-specific
Unmixing View Option
control blocks are shown
in Fig. 270 and Fig. 271.
Any changes done with this View Option control
blocks are effective immediately.
The Image Display in the
Unmixing
View shows 3 panels: the intensity-over-lambda diagram with the
extraction bands (32-channel-LSM 710 and LSM 780 systems only), the table with the intensities in each
ROI over lambda, and the image display (see Fig. 272). The spectra from images which are acquired on an
LSM 700 are shown as integrated spectra. This reflects the way, how Lambda stacks are acquired with
the LSM 700 (see the respective description in the LSM 700 Manual).
To access the Unmixing analysis view type, load or acquire a Lambda stack data set or a multichannel
image and click on the
Unmixing
tab.
The Unmixing View is available (
Æ
displayed) for all Lambda stacks and for all multi-channel
images acquired on hardware with calibrated detectors. This includes all LSM 710, LSM 780,
LSM 710 NLO, LSM 780 NLO, LSM 7
MP
(if NDD detectors calibrated) and LSM 700 systems.
Detector calibration is a service task. This calibration allows that reference spectra can be
acquired at different detector gain settings than the multi-labeled image acquired for unmixing!
Fig. 270
Unmixing View Option control block
Fig.
271
Unmixing - Extract - View Option
control block
