LSM 710 and LSM 780
MACROS AND VISUAL BASIC
Systems
Macros
Carl Zeiss
02/2010 M60-1-0025
e
11
B - Calibration Matrix
−
Is based on the calibration results of the Basic Calibration
−
Needs to be performed for each objective separately
−
Is for calibrating the collimator position of both collimators RGB + V only for the selected Objective
depending on:
Laser Line, Zoom + EF Filter Position
−
Is for calibrating the BSC Y-position depending on:
Laser Line, Zoom + EF Filter Position
On currently delivered LSM 7
LIVE
Systems, the Calibration Matrix is set up in the factory for
every objective belonging to the system (This is done together with the Basic Calibration).
Without running Calibration Matrix, the positions of the collimators and BSC Y should be ok. This is due
to the Basic Calibration, but the results are not fully optimized.
Installation of the Macro:
If not already done, copy the required files to the following destinations:
File Name
Destination
KolliMatic.lvb C:\ZEN\Macros
PotentialKollimaticConfig.ini C:\ZEN\Bin
KolliMatic_Instructions.pdf C:\ZEN\Macros
Starting the Macro:
•
Start the LSM software and check first, if all
prerequisites have been fulfilled
(see section Prerequisites)
•
Put the appropriate calibration slide (depending
on current objective: immersion or non
immersion) onto the microscope stage
•
Activate all lasers, and using the and focus with
the 488 nm line start scanning and focus onto
the beads
•
Put the appropriate calibration slide (depending
on current objective: immersion or non
immersion) on the microscope stage, start single
scan
•
Use the
Macro
menu of the main LSM software
to
Load
and
Run
the Macro
KolliMatic
(see
Fig. 9)
Please wait a few seconds, the Macro will set up
beam path and all necessary settings
automatically.
Fig. 9
Macro Control Window of the main
LSM Software
