MACROS AND VISUAL BASIC
LSM 710 and LSM 780
Carl Zeiss
Macros
Systems
12 M60-1-0025
e
02/2010
Preparations for using the Calibration Slides:
•
Follow the steps shown in the macro
Sample
and Configuration Setup
window.
•
Use the arrows or the drop down menu in order
to select one track for each available laser line
(no need to select several tracks for the same
laser line), start with a track for the lowest
wavelength laser (i.e. typically the 405 nm laser
line.).
•
Select an area of the bead sample that provides
as many beads as possible, at least 4 (fit into
the green rectangle)
•
In Fig. 10, the Basic Calibration has been
selected (Check box
Basic Calibration:
Requires selecting preferred objective
lens.
), therefore some more tracks are available
•
Use the
Laser Power
as a first option in order
to set the intensity, second option is
Integration Time
.
•
In order to start / stop the scan, just select /
deselect the check box
Continuous Scan
.
•
Focus on the beads.
•
Defocus with the
Collimator
slider until the
beads become line shaped.
•
Focus with the microscope focus drive to get
the vertical lines as thin as possible (Fig. 11).
•
Now use the
Collimator
slider to get a round
spot out of the line (Fig. 12).
•
Repeat these steps for a track with the 488 nm
laser line.
•
After one track for each laser line has been
adjusted, click
Next
.
Fig. 10
Figure Sample and Configuration Setup
Window of the KolliMatic Macro
