MACROS AND VISUAL BASIC
LSM 710 and LSM 780
Carl Zeiss
Macros
Systems
20 M60-1-0025
e
02/2010
5.1.7
Image Match (Scanfield Transformation) Macro
Adjusts the Image match between two scanning imaging systems or a manipulation and a scanning
imaging system.
To adjust the image match, set up a multitracking that creates one channel for each scanning and/or
manipulation system. In case of the LSM
DuoScan
in combination with LSM 7
LIVE
, use a T-PMT to create
an image with the
DuoScan
system.
For LSM 7
MP
with two scanning systems, chose the right beam combiner in the reflector turret when
adjusting the scanfields with the macro. It has to be confirmed, that both laser beams are optimally
aligned to the optical axis of each scanner respectively before adjusting the scanfields. The alignement of
the laser beams to the optical axis is performed by the field service engineer at the set up of the system.
•
Start the Image Match (Scanfield Transformation) Macro (Fig. 21).
•
Use the Image match calibration sample delivered with the system. This sample has a fluorescent layer
and a reflective grid, useful to create images in fluorescent, reflective or transmission light mode.
•
Start continuous scan, and set laser power and gain to appropriate levels. The point scanning system
will be the speed limiting system, so choose a fast scanning speed to get a faster image refresh.
•
Use unidirectional scan to avoid adjustment errors due to bidirectional image jitters.
•
For LSM 7
LIVE
with LSM
DuoScan
set the matching zoom factors with one of the
Match DuoScan
zoom/offset
or
Match LIVE zoom/offset
buttons relative to each other. It is recommended to
adjust the LSM zoom.
These buttons set the two scanheads at a matching scanfield diameter.
Fig. 21
Scanfield Transform macro interface
