ANNEX
LSM 710 and LSM 780
Carl Zeiss
Beam path Configuration Guide for …
Systems
8 M60-1-0025
e
02/2010
b) Multiple tracks – sequential acquisition of
channels
Multi-tracking is the method of choice for multi-
fluorescence imaging. It allows avoiding artifacts
from emission crosstalk that can occur in
simultaneous excitation and detection of multiple
dyes. To sequentially acquire multiple channels,
laser lines are switched very fast and channels are
recorded quasi-simultaneously.
The configuration of multiple tracks follows the
same rules described above for a single track. The
main difference is that each track is configured to
excite and detected only one fluorescent dye to
prevent cross talk (or two dyes with non
overlapping emission spectra).
•
Create a track for Alexa 488.
•
Add a new track by clicking the
button.
•
Create a second track for Cy 3.
•
By selecting the dyes from the pulldown menu,
you can see the emission spectra to set the
matching detection bands.
Fig. 2
Image Setup and Light path tools
