MULTIPHOTON LASER SCANNING MICROSCOPY …
LSM 710 and LSM 780
Carl Zeiss
NLO (Non Linear Optics) Laser for LSM …
Systems
2 M60-1-0025
e
02/2010
8
MULTIPHOTON LASER SCANNING MICROSCOPY WITH LSM FAMILY SYSTEMS
8.1
NLO (Non Linear Optics) Laser for LSM 710 NLO, LSM 780 NLO and LSM 7
MP
The LSM 710 NLO, LSM 780 NLO and LSM 7
MP
scanning module are operated with a Titanium-
Sapphire-Laser to perform Multiphoton Microscopy. Depending on the type of laser the following
operation procedures apply:
•
The operation of software controlled lasers (Chameleon laser from Coherent or Mai Tai laser from
Spectra Physics) is integrated into the LSM software.
−
Both lasers should be kept in Stand by mode for daily or weekly operation. The cooling of the laser
must not be shut down during stand by. Only in case of more than two weeks without operation
the laser should be shut down completely. This has to be performed according to the operation
manual for the laser provided by the manufacturer. The shut down and the start procedure can
take up to one hour.
−
Specific for the operation of Chameleon lasers (Coherent; for further details please refer to the
operators manual of the laser):
The key on the front panel of the laser power supply is used for switching between Stand by mode
and Operation mode.
The laser has to be in Operation mode in order to control it with the LSM software.
−
Specific for the operation of Mai Tai lasers (Spectra Physics; for further details please refer to the
operators manual of the laser):
The laser is put to
Operation
mode or Stand by mode using the LSM software. Therefore no
further operation of the laser via the laser power supply and electronics unit is needed. The key on
the front panel of the laser power supply can also be used to put the system from Standby to ON. It
is also an interlock key. When the key is removed the system cannot be operated. The key needs to
be in ON position to be able to control the laser with the software. The power switch on the laser
electronics has to be kept
On
for leaving the laser in Standby.
•
Operation of Verdi Mira lasers (Coherent)
These lasers are not operated via the ZEN software. Refer to the operator manual of the manufacturer
for using these lasers in combination with the LSM 710 NLO, LSM 780 NLO or LSM 7
MP
. However it
is needed to indicate the tuned wavelength in the software to ensure the appropriate setting of the
AOM frequency (as described below in section 8.1.1).
