MULTIPHOTON LASER SCANNING MICROSCOPY …
LSM 710 and LSM 780
Carl Zeiss
NLO (Non Linear Optics) Laser for LSM …
Systems
4 M60-1-0025
e
02/2010
8.1.2
Imaging Setup and Light Path tool
Application of the NLO laser requires special main dichroic beam splitters to be activated in the
Light
Path
tool.
NLO-excited signals can be detected in every channel. However, it is important to efficiently block NIR
(near infrared) excitation stray light from the detector.
The following filters are designed for detection of NLO-excited fluorescence signals:
−
MBS 690+:
main dichroic beam splitter reflecting NIR excitation longer than 690 nm, transmitting
shorter wavelengths
−
MBS -405/760+:
main dichroic beam splitter reflecting NIR excitation longer than 760 nm,
transmitting shorter wavelengths and reflecting 405 nm excitation for simultaneous detection of
signals from NIR and 405 excited fluorochromes.
8.1.3
Pinhole and Collimator Settings
•
The pinhole can be fully opened for maximum detection efficiency due to the intrinsic optical
sectioning capabilities of the NLO effect (see
Channel window
).
•
In the
Pinhole & Collimator Control
window, the
NIR
collimator can be used to align the overlap of
the excitation planes within the object for VIS as well as NIR excitation light in the
Collimator
panel
(see
Maintain
menu,
Pinhole
button).
