CONFOCOR
3
ConfoCor 3
Carrier Position
Carl Zeiss
02/2010 M60-1-0025
e
17
Proceed as follows:
•
Make sure that the front lens of the water
immersion objective is wetted by a drop of
water. Use fluorescence-free double-distilled
water, since otherwise the immersion water will
cause background fluorescence and deteriorate
the correlation signal.
•
Choose a chamber by clicking on one well in
the
X, Y (Stage)
panel of the
Sample Carrier
tool.
•
Set up a beam path in the LSM
Light Path
tool
(Fig. 14).
Convenient settings are:
−
488 nm line at 50 % tube current and 5 %
AOTF
−
MBS T80/R20 major dichroic beam splitter
for Visible light
−
Plate major dichroic beam splitter for
Invisible light
−
None for side port systems, Rear for rear port
systems <t the reflector position
−
Reflection check box activated
−
Appropriate spectral band in the detector
channel to detect the reflected excitation
light, for example 420 – 650 nm for 488 nm
light
Do not use the APD detectors in the
reflected mode to find the coverglass
surface. They might be deteriorate due to
high light intensity and will switch off due to
overload.
Fig. 14
Beam path configuration for
finding glass reflection
