CONFOCOR
3
Carl Zeiss
Carrier Position
ConfoCor 3
18 M60-1-0025
e
02/2010
•
Set up scan mode in the LSM
Acquisition
Mode
tool (Fig. 15). Choose
Line
scan, highest
Scan speed, 512 pixels and
Zoom 1
for
convenience.
•
Set up scan control in the LSM
Channels
tool
(Fig.
16). Open Pinhole to maximum. Set
detector gain between 200 and 300, and laser
power to 2 %.
Fig. 15
Image settings for line scan mode
Fig. 16
Channel settings for finding glass
reflection