CONFOCOR
3
ConfoCor 3
Carrier Position
Carl Zeiss
02/2010 M60-1-0025
e
19
•
Press
Continuous
to start a continuous scan.
Press the
Line Scan
register (Fig. 17).
•
The diagram (Fig. 18) shows a line with a bump,
which is the result of reflected light on the
optical axis not focused yet on the lower glass
surface You might adjust the line to lower or
higher values by altering the gain or laser
power.
•
Move the objective cautiously upward by
turning the Z focus knob of the microscope
stand. A lens moving upward will be indicated
by increasing numbers in the position field of
the
Z (Focus)
panel window.
•
If the focus position approaches the lower cover
slip surface, the position of the line will shift to
higher values (Fig. 19).
Fig. 17
Selection of Line Scan diagram
Fig. 18
Selection of Line Scan diagram
Fig. 19
Line diagram near the lower cover
slip surface
