CONFOCOR
3
Carl Zeiss
Carrier Position
ConfoCor 3
20 M60-1-0025
e
02/2010
•
Continue cautiously moving upwards. The line
position will reach its maximum (Fig. 20).
•
Having cautiously moved over a short distance
the line position will decline again to a
minimum and than rise again to a second
maximum (Fig. 21), which corresponds to the
upper cover slip surface.
•
Store the reached position using the
Store
button.
•
Now you can position the detection volume into
the sample in a well-defined way:
If you use one of the C-Apochromat water
immersion objectives, position the detection
volume 150 - 200 µm deep into the sample to
get rid of disturbing interface effects. The
easiest way of doing this is by clicking the
+200
µm
button.
Fig. 20
Line diagram at lower cover slip
surface
Fig. 21
Line diagram at upper cover slip
surface
