CONFOCOR
3
ConfoCor 3
System Configuration
Carl Zeiss
02/2010 M60-1-0025
e
29
Procedure for setting the laser power
•
Open the
Laser
tool by clicking the
Laser
tab in
the
Setup Manager
tool group (Fig. 33). Close
the tool box by clicking again. The selections
On
the laser to be switched on, if it is controlled
by the software. Switch the requested lasers on
by using the pull down menus. The Argon laser
has to be switched on and controlled offline.
The HeNe lasers and Diode lasers will switch on
automatically after Software start. For each laser
the properties are displayed of the
Laser
Properties
window (Fig. 34) is opened.
•
Set the laser attenuation using the sliders in
Measurement / Pinhole Adjustment
from
the
Laser
control panel in the
Measurement
Tool of the
ConfoCor
tool box. For this
purpose, open the
Countrate
window by
clicking the
Countrate
button in the
ConfoCor
tool group. A good starting point is to set the
intensity in such a way that a count rate
between 50 kHz and 200 kHz is obtained.
•
For most dyes, the Counts / Molecule setting
should be optimized in a second step to a value
just under its maximum by changing the laser
power. If carriers of different slide thickness are
employed the Counts / Molecule setting should
be optimized by using the correction ring on
the lens. The correction ring is turned
counterclockwise or clockwise until a maximum
value is obtained. The correction ring should
also be used for adjusting the Counts
/
Molecule setting whenever the immersion
media is changed. This is especially important in
cases where the refractive index of the
immersion media is different from that of the
sample. When finished, close the
Count Rate
window using the
Close
button.
Fig. 32
Laser lines window
Fig. 33
Laser tool window
Fig. 34
Laser Information window
