CONFOCOR
3
ConfoCor 3
Acquisition
Carl Zeiss
02/2010 M60-1-0025
e
35
9.7
Acquisition
The Acquisition panel allows you to define the
times and positions for your measurement.
•
Click on the
Acquisition
register to open the
Acquisition
panel (Fig. 38). Click on any other
register to leave the panel.
You have three submenus available:
−
Times
: Used to define measurement times and repetitions.
−
Kinetics
: Used to define delay times between consecutive measurements.
−
Position
: Used to define the measurement positions.
9.7.1.1
Times
In the
Times
panel you can set the values for the
Bleach Time
,
Measure Time
and
Repeat Count
(Fig. 39).
•
Click on the
Times
tab to open the
Times
panel (Fig. 39). Click again to close the panel.
Bleach Time (s)
The
Bleach Time
is the time prior to the measuring procedure during which the laser already has an
effect on the sample. It is used to pre-bleach the sample, for example to bleach away immobile molecules
that would not contribute to the correlation and would negatively affect the correlation amplitude.
The
Bleach Time
is taken into consideration only
once
(at the beginning of a measuring cycle). Enter a
number in seconds in the input box or use the scroll arrows.
Note that the laser power set under
Bleach
in the
System Configuration Laser panel
will be
used for the bleach. If no bleach laser was activated but a bleach time entered, the system gives a
warning. Acknowledge if you want to continue anyway.
Measure Time (s)
The
Measure Time
is the period of
one
measurement. As a rule of thumb the measurement time should
be about 1000 x the diffusion time of a molecule under study. However, due to noise in the system a 10
s measurement time is a good start value to obtain good statistical results. Enter a number in seconds in
the input box or use the scroll arrows.
Note that the laser power set under
Measurement / Pinhole Adjust
in the
System Configu-
ration Laser
panel will be used for the measurement. If no laser was activated but a measurement
time entered, the system gives a warning. Acknowledge if you want to continue anyway.
Fig. 38
Acquisition panel
Fig. 39
Times panel
